Ct值计算. CT值的计算公式CT值 Ux-U水 在73千电子伏U水在73千电子伏K式中K是一常数其值等于1000.
ΔCTcalibrator CTtarget calibrator CTref calibrator 其次用校准样本的ΔCT 值归一试验样本的ΔCT 值 ΔΔCT ΔCTtest ΔCTcalibrator 最后计算表达水平比率 2ΔΔCT 表达量的比值.
Normalizeeach ChIP DNA fractions Ct value to the Input DNA fraction Ct valuefor the same qPCR Assay ΔCt to account forchromatin sample preparationdifferencesΔCt Ct - Ct -Log2 Input Dilution FactorWhere Inp.
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